Kwiimuvi ezininzi kunye nemisebenzi yoncwadi, iilabhoratri ze-forensic zibonakala njengobomi obukhethekileyo nobubalulekileyo, ingakumbi ibali lovavanyo lokuchongwa kwe-DNA lidla ngokuba sisitshixo sokufumana izikhokelo nokusombulula amatyala.Nangona kunjalo, ukuba ukuchaneka kweziphumo zovavanyo ezivezwayo kuyathandabuzeka, ngokwemvelo akuyi kuba bubungqina obusemthethweni, ingasathethwa ke ngokutyhila inyaniso kwiikona ezifihlakeleyo. Kukho into ekhethekileyo ebonakalayo neyekhemikhali ekufuneka iilabhoratri ze-forensic ziyiphathe ngononophelo, kwaye oko kukuthintela ukuba iisampulu ze-DNA zivavanywe ekubeni zingcoliswe kwilabhoratri. Okwangoku, kukho izizathu ezahlukeneyo zongcoliseko lwe-DNA. Phakathi kwazo, amathuba okuba kungcoliswe iintsholongwane angaphezulu kakhulu.

Njengakwezinye iilabhoratri, izinto ezisetyenziswayo kunye nezixhobo kwiilabhoratri ze-forensic zingcolisiwe kwiimeko ezininzi. Ngokukodwa, usulelo oludibeneyo phakathi kweesampulu ze-DNA olusekelwe kwiimpendulo ze-PCR, ezinye izixhobo zovavanyo kunye nobungqina obubonakalayo, kunye nabavavanyi ngokwabo zezona zinto zinzima ukuzibona. Ezi ntsalela zongcoliseko ziquka iiseli zebhayoloji, igazi, izicubu, kunye nee-reagents zokuvavanya, iisepha zokuhlamba izitya kunye nezinye izinto ezingcolileyo.

Kufanelekile ukugxininisa ukuba izinto zeglasi ezinokuphinda zisetyenziswe kwiilabhoratri ze-forensic ezifana nezikhongozeli zeesampuli, iibhotile ze-reagent, iityhubhu zovavanyo, ii-pipettes, iiflasks, izitya ze-petri, njl. Ukuphunyezwa kwazo okunganelanga, ukungathotyelwa, kunye nokungathotyelwa kwazo kwimisebenzi yokuhlamba zezinye zezona zinto zibangela ukuba kuchongwe kwaye kuhlalutywe izigqibo ezingalunganga.

Olu hlobo longcoliseko lwezitya zeglasi luyingxaki ecacileyo kwiziphumo zovavanyo, ngoko ke yintoni eyona nto ibalulekileyo ekusombululeni oku?

Okokuqala, xa kufunyenwe imeko erhanelwayo yokungcoliswa kwe-DNA, iziphumo zovavanyo kufuneka zihlolwe kwakhona ngexesha ukuze kubuyiselwe impazamo. Le yeyona nto iphambili.

Emva koko, yenza umkhondo wolawulo lomgangatho kwizinto ezisetyenziswayo zovavanyo, kuquka izikhongozeli zeglasi, ii-reagents, njl.njl., ukuqinisekisa ngakumbi umthombo wongcoliseko.

Ngokwale ndlela, phucula iinkqubo zokucoca izitya zeglasi ukuze kulungiswe iimpazamo, ukuze kuthintelwe ukwenzeka kweempazamo ezifanayo kwakhona.

Okwesithathu, kuphela ngokuqinisa amanyathelo okulwa nongcoliseko kunye nokucoca ilabhoratri ukuze kwenziwe ulawulo olucwangcisiweyo apho ukuphuculwa kwenkqubo yokucoca kunokuba nentsingiselo kwaye kusebenze.

Enyanisweni, ilebhu yophando lwenzululwazi efanelekileyo kufuneka ibe nendawo ezinikeleyo nezimeleyo yovavanyo lwe-DNA ukuqinisekisa ukuba ungcoliseko luyancitshiswa kwiikhonkco ezahlukeneyo zovavanyo. Umzekelo, indawo yokwamkelwa kwetyala kunye nendawo yokugcina isampuli, indawo yokukhupha i-DNA yesampuli, indawo yokwandisa i-DNA, indawo yokufumanisa i-DNA, indawo yokuhlolwa kwangaphambili, indawo yohlalutyo lweziphumo, indawo yokulungiselela, indawo yokwandisa i-DNA, indawo yokufumanisa, njl. Phakathi kwazo, ukucoca izitya zeglasi kwindawo yokulungiselela kuya kunceda kakhulu ukwandisa amathuba okungaphumeleli kweziphumo zovavanyo.

Kufuneka kuqatshelwe ukuba iilabhoratri ezininzi zobungcali, kuquka iilabhoratri ze-forensic, zisasebenzisa iindlela zokucoca ngesandla ezingasebenzi kakuhle ukusombulula ingxaki yokungcola okufumaneka kwiiglasi. Kodwa le ndlela ayiphuculi ngokusisiseko umngcipheko wabantu wokungcola okusasazekayo.

Ngaphezu koko, iingxaki zokucoca izinto zeglasi ngesandla zingaphezulu kakhulu koku.

MUkucocwa kweglasi rhoqo ngonyaka akuyi kuphelisa nje kuphela ukucoca ngokupheleleyo iiglasi zelebhu kwaye kuchaphazele isigqibo sokugqibela sovavanyo lwe-DNA kunye nokuchongwa kwayo, kodwa kuya kuzisa uthotho lweengxabano ezifana nokuchitha izixhobo, ubunzima bokucoca, kunye nemingcipheko yokhuseleko kubasebenzi belebhu. Okwangoku, ukusetyenziswa kwe- umatshini wokuhlamba izinto zeglasi ozenzekelayoezisetyenziswa kwiilabhoratri zamazwe ngamazwe ze-forensic ziya kunceda ukuphucula le meko.

Umatshini wokuhlamba iiglasi zelebhuingacoca ngokuchanekileyo iintlobo ngeentlobo zeglasi zelebhu ngendlela ekhuselekileyo, ebanzi, nekrelekrele ukuze ihambelane nemigaqo efanelekileyo ye-GMP kunye ne-FDA. Xa kuthelekiswa nendlela yokucoca ngesandla, Umatshini wokuhlamba iLebhuinokulandelela inkqubo yokucoca kuyo yonke inkqubo, nto leyo enceda ekufumaneni iirekhodi zedatha ezixabisekileyo kwiinkalo ezahlukeneyo. Ezi datha zibaluleke kakhulu ekususweni kweemeko eziseleyo kuquka ungcoliseko olujongene novavanyo lwe-DNA. Ingakumbi xa kukho umahluko kunye namathandabuzo malunga neziphumo zovavanyo!

Ngophuhliso oluqhubekayo lwetekhnoloji efanelekileyo, iilabhoratri ze-forensic ziya kuthatha uxanduva olungakumbi kwinkqubo yokuphathwa kwamatyala. Ngale ndlela, iimfuno zazo naziphi na iziphumo zovavanyo lwelabhoratri kunye nokubekwa emgangathweni kunye nokuchaneka ziya kuqhubeka ngokwanda. Iindlela eziquka uvavanyo lwe-DNA zinokuqinisekisa kuphela iziphumo ezicocekileyo kunye nokufumana izigqibo ezichanekileyo kunye nobungqina ukuba ziphumelele ekucoceni ukungcola. Le yinto ekufuneka ikhunjulwe yilabhoratri nganye ye-forensic.