I-Anti-cross-contamination, uvavanyo lwe-DNA oluthembekileyo lunokutyhila inyaniso kwiikona ezifihliweyo

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Kwimiboniso bhanyabhanya kunye nemisebenzi yoncwadi, iilabhoratri zophando-nzulu zibonakala njengobukho obukhethekileyo nobubalulekileyo, ngakumbi icebo lovavanyo lokuchongwa kwe-DNA lidla ngokuba sisitshixo sokufumana imikhondo nokusombulula amatyala.Nangona kunjalo, ukuba ukuchaneka kweziphumo zovavanyo ezibonisiweyo kuyathandabuzeka, ngokwemvelo ayiyi kuba bubungqina obusemthethweni, singasathethi ke ngokutyhila inyaniso kwiikona ezifihliweyo.Kukho umba okhethekileyo womzimba kunye neekhemikhali ekufuneka iilabhoratri zophando ziwuphathe ngononophelo, kwaye oko kukuthintela iisampulu ze-DNA eziza kuvavanywa ukuba zingcoliseke elabhoratri.Okwangoku, kukho izizathu ezahlukeneyo zokungcoliseka kweDNA.Phakathi kwabo, ukuba kunokwenzeka ukungcoliseka komnqamlezo ngowona mkhulu.
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Njengezinye iilabhoratri, izinto ezisetyenziswayo kunye nezixhobo kwiilabhoratri zophando zingcolisekile kwiimeko ezininzi.Ngokukodwa, ukosuleleka phakathi kweesampulu ze-DNA ezisekwe kwimpendulo ye-PCR, ezinye izinto zovavanyo kunye nobungqina obuphathekayo, kunye nabavavanyi ngokwabo ngabona banzima kakhulu ukubhaqa.Ezi ntsalela zosulelo ziquka iiseli zebhayoloji, igazi, izicubu, kunye novavanyo lwezixhobo ezisebenza ngokusebenza kwakhona, izicoci zokuhlamba izitya kunye nobunye ukungcola.

Kufanelekile ukugxininisa ukuba i-glassware ephinda isetyenziswe kwiilabhoratri ze-forensic ezifana nezikhongozelo zesampuli, iibhotile ze-reagent, iityhubhu zovavanyo, iipayipi, iiflasks, izitya zepetri, njl njl. amatyala akhokelela ekuchongeni okungalunganga kunye nezigqibo zokuhlalutya.

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Olu hlobo longcoliseko lwe-glassware luyisongelo esicacileyo kwiziphumo zovavanyo, ngoko yintoni isitshixo sokuyisombulula?

Okokuqala, xa imeko ekrokrelekayo yongcoliseko lwe-DNA ifunyenwe, iziphumo zovavanyo kufuneka ziqwalaselwe kwakhona kwangethuba ukuze kubuyiselwe impazamo.Le yeyona nto iphambili.

Emva koko, qhuba umkhondo wolawulo lomgangatho kwizinto ezisetyenziswayo zokulinga, kubandakanya nezikhongozeli zeglasi, ii-reagents, njl.njl., Ukuqinisekisa ngakumbi umthombo wosulelo.

Ngesi siseko, phucula iinkqubo zokucoca i-glassware ukulungisa iimpazamo, ukwenzela ukuba ugweme iimpazamo ezifanayo ukuba zingenzeki kwakhona.

Okwesithathu, kuphela ngokomeleza imilinganiselo yokuchasana nokungcoliseka kwendalo kunye nokuphelisa ungcoliseko lwebhubhoratri ukwenza ulawulo lwamaziko apho ukuphuculwa kwenkqubo yokucoca kunokuba nentsingiselo kwaye kusebenze.

Enyanisweni, ilabhoratri yophando olusemthethweni kufuneka ibe nendawo ezinikeleyo nezimeleyo yovavanyo lwe-DNA ukuqinisekisa ukuba ungcoliseko luyacuthwa kumakhonkco ovavanyo ahlukeneyo.Ngokomzekelo, ukwamkelwa kwamatyala kunye nendawo yokugcina isampula, indawo yokukhutshwa kwesampula ye-DNA, indawo yokukhulisa i-DNA, indawo yokukhangela i-DNA, indawo yokuhlola kwangaphambili, indawo yokuhlalutya iziphumo, indawo yokulungiselela, indawo yokukhulisa i-DNA, indawo yokukhangela i-buffer, njalo njalo.Phakathi kwabo, ukucoca i-glassware kwindawo yokulungiselela kuya kunceda kakhulu ukwandisa amathuba okungaphumeleli kweziphumo zovavanyo.

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Kufuneka kuqatshelwe ukuba iilebhu ezininzi zeengcali, kubandakanywa iilabhoratri ze-forensic, zisasebenzisa iindlela ezingasebenziyo zokucoca ngesandla ukusombulula ingxaki yeentsalela zokungcola kwi-glassware.Kodwa le ndlela ayiphuculi ngokusisiseko umngcipheko wosulelo lwabantu.

Ngaphezu koko, izinto ezingalunganga zokucocwa ngesandla kweglasi zihamba ngaphaya koku.

MUkucocwa kweglasi yeglasi akuyi kusilela kuphela ukucoca ngokucokisekileyo i-glassware yelabhoratri kwaye kuchaphazele ukuqukunjelwa kokugqibela kovavanyo lwe-DNA kunye nokuchongwa, kuya kuzisa nothotho lwezichasi ezinje ngenkcitho yemithombo, ubunzima bemisebenzi yokucoca, kunye nemingcipheko yokhuseleko kubasebenzi basebhubhoratri. .Ngeli xesha, ukusetyenziswa kwe iwasha glassware oluzenzekelayoezisetyenziswa kwiilebhu zamazwe ngamazwe zophando-nzulu ziya kunceda ekuphuculeni le meko.

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Iwasha yeglasi yelabhoratriinokucoca ngokuchanekileyo i-glassware eyahlukahlukeneyo yelabhoratri ngendlela ekhuselekileyo, ibhetshi, kunye nengqiqo yokwenza ukuba ihambelane nemimiselo efanelekileyo ye-GMP kunye ne-FDA.Xa kuthelekiswa nendlela yokucoca ngesandla, i Iwasha yeLabhoratriinokulandelela inkqubo yokucoca kuyo yonke inkqubo, enceda ekufumaneni iirekhodi zedatha ezibalulekileyo kwiinkalo ezahlukeneyo.Ezi nkcukacha zibaluleke kakhulu ekususweni kweemeko ezishiyekileyo kuquka ungcoliseko olujongene novavanyo lwe-DNA.Ngokukodwa xa kukho iiyantlukwano kunye namathandabuzo malunga neziphumo zovavanyo!

Ngophuhliso oluqhubekayo lweetekhnoloji ezifanelekileyo, iilabhoratri ze-forensic ziya kuthatha uxanduva ngakumbi kwinkqubo yokusingatha iimeko.Ngale ndlela, iimfuno zazo naziphi na iziphumo zovavanyo lwebhubhoratri kunye nokubekwa emgangathweni kunye nokuchaneka ngokwemvelo kuya kuqhubeka ukwanda.Iindlela ezibandakanya uvavanyo lwe-DNA zinokuqinisekisa kuphela iziphumo ezicocekileyo kwaye ufumane izigqibo ezichanekileyo kunye nobungqina ukuba ziphumelele ekukhupheni ukungcola.Le yinto ekufuneka ikhunjulwe yilabhoratri yophando lwenkundla.


Ixesha lokuposa: Apr-02-2021